top of page

Publications

2024

Kim JE, Pan X, Tse KY, Chan HH, Dong C, Huen MS. PHF8 facilitates transcription recovery following DNA double-strand break repair. Nucleic Acids Res 2024; 52(17): 10297-10310.

Li J, Xiong N, West KL, Leung M, Ching YP, Huang J, Yuan J, Yu CH, Leung JW, Huen MS. Nuclear F-actin assembly on damaged chromatin is regulated by DYRK1A and Spir1 phosphorylation. Nucleic Acids Res 2024; 52(15): 8897-8912.

Ng RR, Lin Z, Zhang Y, Ti SC, Javed A, Wong JW, Fang Q, Leung JW, Tang AH, Huen MS. R-loop resolution by ARIP4 helicase promotes androgen-mediated transcription induction. Sci Adv 2024; 10(29): eadm9577.

Lee HH, Chow KL, Wong HS, Chong TY, Wong AS, Cheng GH, Ko JM, Siu HC, Yeung MC, Huen MS, Tse KY, Bray MR, Mak TW, Leung SY, Ip PP. Inhibition of aberrantly overexpressed Polo-like kinase 4 is a potential effective treatment for DNA damage repair-deficient uterine leiomyosarcoma. Clin Cancer Res 2024; 30(17): 3904-3918.

Wu J, Chen Y, Li R, Guan Y, Chen M, Yin H, Yang X, Jin M, Huang B, Ding X, Yang J, Wang Z, He Y, Wang Q, Luo J, Wang P, Mao Z, Huen MS, Lou Z, Yuan J, Gong F. Synergistic anticancer effect by targeting CDK2 and EGFR-ERK signaling. J Cell Biol 2024; 223(1): e202203005.

2023

Man CH, Lam W, Dang CC, Zeng XY, Zheng LC, Chan NN, Ng KL, Chan KC, Kwok TH, Ng TC, Leung WY, Huen MS, Wong CC, So CWE, Dou Z, Goyama S, Bray MR, Mak TW, Leung AY. Inhibition of PLK4 remodels histone methylation and activates the immune response via the cGAS-STING pathway in TP53-mutated AML. Blood 2023; 142(23): 2022-2015.

2022

Fang H, Yan HH, Bilardi RA, Flensburg C, Yang H, Barbour JA, Siu HC, Turski M, Chew E, Xu Z, Lam ST, Sharma R, Xu M, Li J, Ip HW, Cheung CY, Huen MS, Sweet-Cordero EA, Majewski IJ, Leung SY, Wong JW. Ganciclovir-induced mutations are present in a diverse spectrum of post-transplant malignancies. Genome Med 2022; 14(1): 124.

 

Ding L, Luo Y, Tian T, Chen X, Yang Y, Bu M, Han J, Yang B, Yan H, Liu T, Wu M, Zhang G, Xu Y, Zhu S, Huen MS, Mao G, Huang J. RNF4 controls the extent of replication fork reversal to preserve genome stability. Nucleic Acids Res 2022; 50(10): 5672-5687.

2021

Feng D, Wang J, Yang W, Li J, Lin X, Zha F, Wang X, Ma L, Choi NT, Mii Y, Takada S, Huen MS, Guo Y, Zhang L, Gao B. Regulation of Wnt/PCP signaling through p97/VCP-KBTBD7-mediated Vangl ubiquitination and endoplasmic reticulum-associated degradation. Sci Adv 2021; 7(20): eabg2099.

Han J, Wan L, Jiang G, Cao L, Xia F, Tian T, Zhu X, Wu M, Huen MS, Wang Y, Liu T, Huang J. ATM controls the extent of DNA end resection by eliciting sequential posttranslational modifications of CtIP. PNAS 2021; 118(12): e2022600118.

 

Dong C, An L, Yu CH, Huen MS. A DYRK1B-dependent pathway suppresses rDNA transcription in response to DNA damage. Nucleic Acids Res 2021; 49(3): 1485-1496.

 

Ding D, Sun X, Pang MY, An L, Huen MS, Hu T, Ishibashi T. RECQL5 KIX domain splicing isoforms have distinct functions in transcription repression and DNA damage response. DNA Repair 2021; 97: 103007.

 

Che N, Ng KY, Wong TL, Tong M, Kau PW, Chan LH, Lee TK, Huen MS, Yun JP, Ma S. PRMT6 deficiency induces autophagy in hostile microenvironment of hepatocellular carcinoma tumors by regulating BAG5-associated HSC70 stability. Cancer Lett 2021; 501: 247-262.

2020

Dong C, West KL, Tan XY, Li J, Ishibashi T, Yu CH, Sy SM, Leung JW, Huen MS. Screen identifies DYRK1B network as mediator of transcription repression on damaged chromatin. PNAS 2020; 117(29): 17019-19030.

Ng RR, Huen MS. Deacetylation of a deacetylase drives the DNA Damage Response. Genome Instab Dis 2020; 1: 151-154.

 

Li M, Feng H, Lin Z, Zheng J, Liu D, Guo R, Li J, Li RHW, Ng RH, Huen MS, Wang PJ, Yeung WS, Liu K. The novel male meiosis recombination regulator coordinates the progression of meiosis prophase I. J Genet Genomics 2020; 47(8): 451-465.

 

Tan XY, Huen MS. Perfecting DNA double-strand break repair on transcribed chromatin. Essays in Biochemistry 2020; EBC20190094.

 

Wan YC, Leung TC, Ding D, Sun X, Lui J, Zhu L, Kang TZ, Yang D, Zhang Y, Zhang J, Qian C, Huen MS, Li Q, Chow MZ, Zheng Z, Han J, Goel A, Wang X, Ishibashi T, Chan KM. Cancer-associated histone mutation H2BG53D disrupts DNA-histone octamer interaction and promotes oncogenic phenotypes. Signal Transduct Target Ther 2020; 5(1): 27.

Chen J, Li P, Song L, Bai L, Huen MS, Liu Y, Lu LY. 53BP1 loss rescues embryonic lethality but not genomic instability of BRCA1 total knockout mice. Cell Death Diff 2020; 27(9): 2552-2567.

Sy SM, Guo Y, Lan Y, Ng H, Huen MS. Preemptive homology-directed DNA repair fosters complex genomic rearrangements in hepatocellular carcinoma. Transl Oncol 2020; 13(9): 100796.

2019

West KL, Kelliher JL, Xu Z, An L, Reed MR, Eoff RL, Wang J, Huen MS, Leung JW. LC8/DYNLL1 is a 53BP1 effector and regulates checkpoint activation. Nucleic Acids Res 2019; 47(12): 6236-6249.

 

Roewenstrunk J, Di Vona C, Chen J, Borras E, Chao D, Arato K, Sabido E, Huen MS, de la Luna S. A comprehensive proteomics-based interaction screen that links DYRK1A to RNF169 and to the DNA damage response. Sci Rep 2019; 9(1): 6014.

2018

Chen Y, Li J, Cao F, Lam J, Cheng CC, Yu CH, Huen MS. Nucleolar residence of the Seckel Syndrome protein TRAIP is coupled to ribosomal DNA transcription. Nucleic Acids Res 2018; 46(19): 10119-10131.

 

Chan LH, Zhou L, Ng KY, Wong TL, Lee TK, Sharma R, Loong JH, Ching YP, Yuan YF, Xie D, Lo CM, Man K, Artegiani B, Clevers H, Yan HH, Leung SY, Richard S, Guan XY, Huen MS, Ma S. PRMT6 regulates RAS/RAF binding and MEF/ERK-mediated cancer stemness activities in hepatocellular carcinoma through CRAF methylation. Cell Rep 2018; 25(3): 690-701.

 

An L, Dong C, Li J, Chen J, Yuan J, Huang J, Chan KM, Yu CH, Huen MS. RNF169 limits 53BP1 deposition at DSBs to stimulate single-strand annealing repair. PNAS 2018; 115(35): E8286-8295.

 

Jiang J, Tang S, Xia J, Wen J, Chen S, Shu X, Huen MS, Deng Y. C9orf140, a novel Axin1-interacting protein, mediates the negative feedback loop of Wnt/beta-catenin signaling. Oncogene 2018; 37(22): 2992-3005.

 

Liu J, Lv L, Gong J, Tan Y, Zhu Y, Dat Y, Pan X, Huen MS, Li B, Tsao SW, Huo J, Cheung AL. Overexpression of F-box only protein 31 predicts poor prognosis and deregulates p38a- and JNK-mediated apoptosis in oesophageal squamous cell carcinoma. Int J Cancer 2018; 142(1): 145-155. 

2017

Han J, Ruan C, Huen MS, Wang J, Xie AY, Fu C, Liu T, Huang J. BRCA2 antagonizes classical and alternative non homologous end-joining to prevent gross genomic instability. Nat Commun 2017; 8(1): 1470.

 

Lou J, Chen H, Han J, He H, Huen MS, Feng XH, Liu T, Huang J. AUNIP/C1orf135 directs DNA double-strand breaks towards the homologous recombination repair pathway. Nat Commun 2017; 8(1): 985. (Recommended by F1000Prime)

 

An L, Jiang Y, Ng HH, Man EP, Chen J, Khoo US, Gong Q, Huen MS. Dual-utility NLS drives RNF169-dependent DNA damage responses. PNAS 2017; 114(14): E2872-2881.

(Recommended by F1000Prime;

Highlighted in Science Signaling - "NLS protects DNA damage protein")

Guo Y, An L, Ng HM, Sy SM, Huen MS. An E2-guided E3 screen identifies the RNF17-UBE2U pair as regulator of the RIDDLE syndrome protein RNF168, J Biol Chem 2017; 292(3): 967-978.

2016

2015

Guo Y, Feng W, Sy SM, Huen MS. ATM-dependent phosphorylation of the Fanconi anemia protein PALB2 promotes the DNA Damage Response. J Biol Chem 2015; 290: 27545-56.

 

Lau WC, Li Y, Zhang QF, Huen MS. Molecular architecture of the Ub-PCNA/Pol eta complex bound to DNA. Sci Rep 2015; 5:15759.

 

Yang J, Deng W, Hau PM, Liu J, Lau VM, Cheung AL, Huen MS, Tsao SW. Epstein-Barr virus BZLF1 protein impairs accumulation of host DNA damage proteins at damage sites in response to DNA damage. Lab Invest 2015; 95(8):937-50.

 

Hodge CD, Edwards RA, Markin CJ, McDonald D, Pulvino M, Huen MS, Zhao J, Spyracopoulos L, Hendzel MJ, Glover JN. Covalent inhibition of Ubc13 affects ubiquitin signaling and reveals active site elements important for target. ACS Chem Biol 2015; 10(7):1718-28.

 

Hau PM, Deng W, Jia L, Yang J, Tsurumi T, Chiang AK, Huen MS, Tsao SW. Role of ATM in the formation of replication compartment during lytic replication of EBV in nasopharyngeal epithelial cells. J Virol 2015; 89(1):652-68.

2014

Dong S, Han J, Chen H, Liu T, Huen MS, Yang Y, Guo C, Huang J. The human SRCAP chromatin remodeling complex promotes DNA-end resection. Curr Biol 2014; 24(18):2097-110.

 

Liu J, Han L, Li B, Yang J, Huen MS, Pan X, Tsao SW, Cheung AL. F-Box Only Protein (FBXO31) negatively regulates p38 Mitogen-Activated Protein (MAP) kinase signaling by mediating lysine48-linked ubiquitination and degradation of MAP Kinase Kinase 6 (MKK6). J Biol Chem 2014; 289(31):21508-18.

 

Han J, Liu T, Huen MS, Hu L, Chen Z, Huang J. SIVA1 directs the E3 ubiquitin ligase RAD18 for PCNA monoubiquitination. J Cell Biol 2014; 205(6):811-27.

2013

2012

Chen J, Feng W, Jiang J, Deng Y, Huen MS. Ring Finger Protein RNF169 antagonizes the ubiquitin-dependent signaling cascade at sites of DNA damage. J Biol Chem 2012; 278(33):27715-22.

 

Guerra-Rebollo M, Mateo F, Franke K, Huen MS, Lopitz-Otsoa F, Rodriguez MS, Plans V, Thomson TM. Nucleolar exit of RNF8 and BRCA1 in response to DNA damage. Exp Cell Res 2012; 318(18):2365-76.

 

Leung JW, Wang Y, Fong KW, Huen MS, Li L, Chen J. Fanconi anemia (FA) binding protein FAAP20 stablizes FA complementation group A (FANCA) and participates in interstrand cross-link repair. PNAS 2012; 109(12):4491-6.

 

Shao Z, Li F, Sy SM, Yan W, Zhang Z, Gong D, Wen B, Huen MS, Gong Q, Wu J, Shi Y. Specific recognition of phosphorylated tail of H2AX by the tandem BRCT domains of MCPH1 revealed by complex structure. J Struct Biol 2012; 177(2):459-68.

 

Liu T, Chen H, Kim H, Huen MS, Chen J, Huang J. RAD18-BRCTx interaction is required for efficient repair of UV-induced DNA damage. DNA Repair 2012; 11(2):131-8.

 

Lok GT, Sy SM, Dong S, Ching YP, Tsao SW, Thomson TM, Huen MS. Differential regulation of RNF8-mediated Lys48- and Lys63-based poly-ubiquitylation. Nucleic Acids Res 2012; 40(1): 196-205.

 

Liu J, Ouyang M, Jiang J, Mu P, Wu J, Yang Q, Zhang C, Xu W, Wang L, Huen MS, Deng Y. Mequindox induced cellular DNA damage via generation of reactive oxygen species. Mutat Res 2012; 741(1-2): 70-5.

2011

Rai R, Li JM, Zheng H, Lok GT, Deng Y, Huen MS, Chen J, Jin J and Chang S. The E3 ubiquitin ligase RNF8 stabilizes TPP1 to promote telomere end protection. Nat Struct Mol Biol 2011; 18(12): 1400-7.

 

Liu J, Ge X, Ouyang M, Tang X, Wu J, Wang J, Jiang J, Huen MS, Deng Y. Catalytic characteristics of CYP3A22-dependent mequindox detoxification. Catal Commun 2011; 12(7): 637-43.

 

Dong S, Huen MS. Roles of histone ubiquitylation in DNA damage signaling. Front Biol 2011; 6(5): 390-7.

 

Hau PM, Yip YL, Huen MS, Tsao SW. Loss of ΔNp63α promotes mitotic exit in epithelial cells. FEBS Lett 2011; 585(17):2720-6.

 

Hau PM, Tsang CM, Yip YL, Huen MS, Tsao SW. Id1 interacts and stabilizes the Epstein-Barr Virus latent membrane protein 1 (LMP1) in nasopharyngeal epithelial cells. PLoS ONE 2011; 6(6):e21176.

 

Chen M, Huang JD, Deng HK, Dong S, Deng W, Tsang SL, Huen MS, Chen L, Zan T, Zhu GX, Guan XY. Overexpression of eIF-5A2 in mice causes accelerated organismal aging by increasing chromosome instability. BMC Cancer 2011; 11:199.

 

Sy SM, Jiang J, Dong S, Lok GT, Wu J, Cai H, Yeung ES, Huang J, Chen J, Deng Y, Huen MS. Critical roles of Ring Finger Protein RNF8 in replication stress responses. J Biol Chem 2011; 286(25): 22355-22361.

2010

Sy MS, Chen J, Huen MS. The 53BP1-EXPAND1 connection in chromatin structure regulation. Nucleus 2010; 1(6):472-4.

 

Deng W, Tsao SW, Mak GW, Tsang CM, Ching YP, Guan XY, Huen MS, Cheung AL. Impact of G(2) checkpoint defect on centromeric instability. Oncogene 2011; 30(11):128-9.

 

Huen MS, Sy SM, Leung KM, Ching YP, Tipoe GL, Man C, Dong S, Chen J. SON is a spliceosome-associated factor required for mitotic progression. Cell Cycle 2010; 9(13):2679-85.

 

Huen MS, Chen J. ATM creates a veil of transcriptional silence. Cell 2010; 141:924-6.

 

Santos MA, Huen MS, Jankovic M, Chen H, Lopez-Contreras A, Klein IA, Wong N, Barbancho JL, Fernandez-Capetillo O, Nussenzweig MC, Chen J, Nussenzweig A. Class switching and meiotic defects in mice lacking the E3 ubiquitin ligase RNF8. J Exp Med 2010; 207(5):973-81.

 

Huen MS, Huang J, Leung JW, Sy SM, Leung KM, Ching YP, Tsao SW, Chen J. Regulation of chromatin architecture by the PWWP domain-containing DNA damage responsive factor EXPAND1. Mol Cell 2010; 37:1-11.

(Recommended by F1000Prime)

 

Huen MS, Sy SM, Chen J. BRCA1 and its toolbox for the maintenance of genome integrity. Nat Rev Mol Cell Biol 2010; 11(2):138-48.

 

Huen MS, Chen J. Assembly of checkpoint and repair machineries at DNA damage sites. Trends Biochem Sci 2010; 35(2):101-8.

Before July 2009

Liu C, Wang Z, Huen MS, Lu LY, Lu DP, Huang JD. Cell death caused by single-stranded oligodeoxynucleotide-mediated targeted genome sequence modification. Oligonucleotides 2009;19(3):218-6.

 

Sy SM, Huen MS, Chen J. MRG15 is a novel PALB2 interacting factor involved in homologous recombination. J Biol Chem 2009; 284(32):21127-31.

 

Sy SM, Huen MS, Zhu YY, Chen J. PALB2 regulates recombinational repair through chromatin association and oligomerization. J Biol Chem 2009; 284(27):18302-10.

 

Sy SM, Huen MS, Chen J. PALB2 is an integral component of the BRCA complex required for homologous recombination repair. PNAS 2009; 106(17):7155-60.

(Highlighted in Science – “Complicated Supercomplexes”)

 

Huang J, Huen MS, Kim H, Leung CC, Glover JN, Yu X, Chen J. RAD18 transmits DNA damage signaling to elicit homologous recombination repair. Nat Cell Biol 2009; 11(5):592-603.

(Recommended by F1000Prime)

 

Huen MS, Chen J. When loose ends finally meet. Nat Struct Mol Biol 2008;15(12):1241-2.

 

Wu J, Huen MS, Lu LY, Ye L, Dou Y, Ljungman M, Chen J, Yu X. Histone ubiquitination associates with BRCA1-Dependent DNA Damage Response. Mol Cell Biol 2009; 29(3):849-60.

 

Huen MS, Huang J, Yuan J, Yamamoto M, Akira S, Ashley C, Xiao W, Chen J. Non-canonical E2 variant-independent function of UBC13 in promoting checkpoint protein assembly. Mol Cell Biol. 2008;28(19):6104-12.

 

Minter-Dykhouse K, Ward I, Huen MS, Chen J, Zou L. Distinct versus overlapping functions of MDC1 and 53BP1 in DNA damage response and tumorigenesis. J Cell Biol 2008;181(5):727-35.

 

Lu LY, Huen MS, Tai AC, Liu DP, Cheah KS, Huang JD. Highly efficient deletion method for the engineering of plasmid DNA with single-stranded oligonucleotides. Biotechniques 2008;44(2):217-24.

 

Huen MS, Sy SM, van Deursen JM, Chen J. Direct interaction between SET8 and PCNA couples H4-K20 methylation with DNA replication. J Biol Chem 2008; 283(17):11073-7.

 

Huen MS, Chen J. The DNA damage response pathways: at the crossroad of protein modifications. Cell Res 2008; 18(1):8-16.

 

Huen MS, Grant R, Manke I, Minn K, Yu X, Yaffe MB, Chen J. RNF8 transduces the DNA-damage signal via histone ubiquitylation and checkpoint protein assembly. Cell 2007; 131(5):901-14.

(Recommended by F1000Prime;

Highlighted in Nat Rev Mol Cell Bio – "DNA damage: Conducting repair"

and in Nat Struct Mol Biol – "DNA damage: ubiquitin marks the spot")

Huen MS, Lu LY, Liu DP, Huang JD. Active transcription promotes single-stranded oligonucleotide mediated gene repair. Biochem Biophys Res Commun 2007;353(1):33-9.

 

Huen MS, Li XT, Lu LY, Watt RM, Liu DP, Huang JD. The involvement of replication in single stranded oligonucleotide-mediated gene repair. Nucleic Acid Res 2006;34(21):6183-94. 

bottom of page